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Considerable losses occur due to browning of fruits and vegetables. Storage and handling costs also increase, if it gets spoiled during processing steps. Browning reduces the quality of fruits and vegetables. It decreases nutritional value and the flavor of fruits and vegetables. When foods like fruits and vegetables are cut or peeled, they release an enzyme from their cells, which is known as phenolase or polyphenol oxidase.

This enzyme is highly reactive in the presence of oxygen. It catalyzes a biochemical reaction that converts the phenolic compounds present in foods to brown pigments known as melanins.

This reaction is known as enzymatic oxidative browning, which renders food the typical black or brown tinge. The enzyme catalyzing enzymatic browning in fruits is polyphenol oxidase or phenolase. In chemistry, there’s a class of phenolic compounds, that are present in fruits and vegetables, known as polyphenols, which form the substrate for this browning enzyme.

Polyphenol oxidase is found in plastids and chloroplasts. They also occur freely in cytoplasm assisting in senescence and browning of fruits and vegetables.

Brunissement enzymatique et non enzymatique pdf – Driver Link Training

Polyphenols can be classified into different categories like anthocyanins, flavonoids, and non-flavonoids. Anthocyanins are pigments present in fruits, flavonoids are occur in catechins, tannins, and brunissemwnt, and non-flavonoids are a component of gallic acid in tea leaves. These polyphenols are unstable and undergo oxidation when exposed to air through a series of biochemical reactions involving conversion of one substrate to a product.


The most common chemical reaction taking place is the catalysis of first step of biochemical reactions involving formation of quinones from phenolic compounds, and subsequent polymerization of quinones to insoluble polymers known as melanin. This compound called melanin is responsible for the browning of fruits and vegetables. Schematic Diagram of Enzymatic Browning.

In this diagram, tyrosine is the phenolic compound that forms the substrate for the enzyme phenoloxidase.

The Maillard Reaction in Food: An Introduction

Secondary reactions and product formations finally lead to the formation of melanin, which aids the browning process. In this biochemical reaction, the enzyme utilizes molecular oxygen as co-substrate. The reaction is dependent on the presence of air and pH level of the fruits.

Acidic pH favors enzymatic browning reaction. Enzymatic Browning of Apples. In apples, the phenolic substrates are present in the flesh and peel. Emzymatique substrates are chlorogenic acid, caffeic acid, 3,4-dihydroxyphenylalanine DOPAp-coumaric acid, flavonol glycosides, 4-methyl catechol, catechin, 3,4-dihydroxy benzoic acid, p-cresol, and leucocyanidin. These compounds are substrates for this browning enzyme, which finally lead to formation of browning products.

It is known that the final product melanin has antimicrobial properties, which prevents any infection and inflammation to the plant or fruits. Melanin also has antibacterial, antioxidant, and anticancer properties. These render the food physiologically wholesome. Enzymatic browning can be prevented by application of several methods. These methods are mostly targeted to inactivate the enzyme and remove oxygen from the product.

Blanching is one of the most effective methods to control enzymatic browning. It involves application of heat for short term to destroy the activity of the enzyme, before freezing or storing fruits and vegetables. Refrigeration and freezing prevents spoilage of food.


Lowering the pH to 4 by adding citric acids and ascorbic acids inhibits the activity of enzymes. Sprinkling lime juice or vinegar prevents this reaction. Dehydration is also one of the most effective methods to prevent enzymatic browning, and various methods enyzmatique irradiation and cold pasteurization are applied to prevent the activity of enzyme phenolase. High Pressure Processing HPP is a technique widely used in the food industry to prevent enzymatic browning reaction.

This involves application of atmospheric pressure to inactivate the enzyme and microbes. Large amount of inhibitors like reducing agents, acidulants, chelating agents, and enzyme inhibitors are used in the food processing industry to prevent the browning effect. Enzymatic browning can cause considerable losses in the food and agricultural sector if necessary measures are not implemented to prevent it.

So, whenever you cut vegetables or fruits, make sure you consume them as soon as possible so that they are not spoiled. How to Calculate Theoretical Yield. Different Branches of Chemistry. Polypropylene Properties and Uses. Enzykatique Dalton’s Atomic Theory.

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